Restriction endonuclease, also known as restriction enzyme, is a class of enzymes isolated from bacteria by W. Arber, H. Smith and D. Nathans et al. (1979). Restriction enzymes have extremely high specificity. They recognize specific sites on double-stranded DNA and cut both strands to form ... Views: 562
Biology needs a metabolic reaction at all times. This is because a special type of catalyst-enzymes are widely present in the body. It can effectively reduce the activation energy of each molecule participating in a chemical reaction, enabling the organism to complete various chemical reactions ... Views: 560
You’ve probably heard the word “enzymes” before, but in such a vaguely scientific context, and you may not know exactly what they are or how they keep you healthy. As it turns out, there are over 70,000 known enzymes in the human body — an astounding number for sure. Enzymes provide the ... Views: 553
People get all the nutrients and energy they need through food. A reasonable diet and adequate nutrition can improve the health of a generation, prevent a variety of diseases, prolong life and improve the physical fitness of the entire nation. Unreasonable diet, excessive or insufficient ... Views: 553
Enzyme biotechnology and industrial enzyme production exist in our daily life. In many cases, commercial processes first utilize naturally occurring enzymes. However, this does not mean that the enzyme can be utilized as effectively as possible. With time, research and improved protein ... Views: 552
Catalase is an antioxidant enzyme that is ubiquitous in all organisms. It is a tetramer (a type of protein) with four polypeptide chains, each containing more than 500 amino acids. It consists of four iron-based (porphyrin heme) reactive with hydrogen peroxide. Catalase is the power house of the ... Views: 552
Thioredoxin reductase (TrxR) is similar in activity to glutathione reductase (GR), catalyzing the reduction of GSSG to GSH, and is one of the key enzymes of the glutathione redox cycle.
TrxR is a dimeric flavoproteinase, a member of the pyridine nucleotide disulfide reductase family, widely ... Views: 550
Although there are thousands of enzymes in the human body, the greatest impact on human nutrition intake is digestive enzymes. The lack of sufficient digestive enzymes not only prevents food nutrients from being fully absorbed and utilized, but also affects the efficiency of muscle synthesis in ... Views: 550
Superoxide dismutase (SOD) is a biological enzyme that protects the body from oxidative damage and has significant protective effects on the body. It is widely distributed in animals, plants and microorganisms. SOD has the ability to scavenge superoxide free O2-in the body, and can better resist ... Views: 548
Nature of the enzyme
Scientifically, metabolism describes all of the biochemical processes that occur in the organisms every day. Metabolic activity is the basis for all life activities and is the guarantee for the continuation of life activities. It is inseparable from the catalytic action ... Views: 544
Enzymes are involved in the formation and composition of every cell in the body. Thus they also control the working of every organ and even the brain - the control center. They are involved in the breakdown, absorption, distribution and circulation of various nutrients entering the body. ... Views: 543
Once upon a time, breast milk was considered the best food for babies. Until now, many people still believe in this. However, even the most perfect food in nature can be contaminated by the earth's ecology destroyed by humans. The pollution and destruction of the ecological environment and the ... Views: 543
If there were no restriction enzymes, what would be the development of molecular biology research today? For 40 years, restriction endonucleases, working behind the scenes, have quietly promoted many basic biological research and commercial applications. Restriction endonucleases (also known as ... Views: 541
Pepsin is one of the main protein degradation or proteolytic enzymes in the digestive system. During the digestion process, pepsin acts on complex dietary proteins and is broken down into peptides and amino acids that are easily absorbed by the intestinal wall. It can be used as an adjuvant ... Views: 540
Cellulase is a protein that can decompose cellulose into oligosaccharides or monosaccharides. It is not a monomeric enzyme, but a synergistic multi-component enzyme system. And it is a complex enzyme composed mainly of exoglucanases.
Cellulase is currently widely utilized in paper, food, ... Views: 540
Cellular activity carrying out in the living body depends on different chemical reactions. If there is no enzyme, then the body's metabolism and other life-related reactions can only be carried out at an extremely slow rate. Under such circumstances, life activities cannot be maintained. ... Views: 539
Dairy products are an indispensable part of daily life for each of us. Strangely, we often hear that someone will have abdominal pain and diarrhea after drinking pure milk, and this reaction is definitely not caused by food spoilage.
There is such a disease called lactose intolerance. People ... Views: 539
As a biocatalyst, enzyme has been widely used in various production fields of light industry. In recent decades, with the continuous technological breakthroughs in enzyme engineering, it has become more and more widely used in industry, agriculture, medicine and health, energy development and ... Views: 535
Enzymes have endless life energy and can be said as the source of human vitality. Enzyme is a protein chemical that can carry the main energy required for each chemical reaction in the human body. Human cells contain about 1,300 kinds of enzymes, which combine with coenzymes to form about ... Views: 534
Although there are thousands of enzymes in the human body, the most significant impact on human nutritional intake is digestive enzymes. The lack of sufficient active digestive enzymes not only prevents food nutrients from being fully absorbed, but also affects the efficiency of muscle synthesis ... Views: 530
Enzymes are molecules that accelerate chemical reactions in biological organisms, including plants, animals, and bacteria. They are often referred to as catalysts because they initiate or accelerate these chemical reactions. In apples, various enzymes are involved in metabolic pathways leading ... Views: 529
Peroxidase, usually derived from horseradish, is a common enzyme used in clinical testing reagents. It is widely used not only in several biochemical assays but also in immunoassay (ELISA) kits. As a key component of the color development system of several kits, peroxidase has an important ... Views: 527
Lack of enzymes, especially lipase deficiency, may cause vascular problems such as cardiovascular disease and high blood pressure. If the lack of lipase occurs in certain parts of the body, it may also cause obesity. It has been reported that obese patients have less lipase in adipose tissue. ... Views: 526
On a global scale, fungal infections are considered to be the main killer. They are hard to detect, and we can't diagnose serious fungal infections every day, and the chances of survival diminish. Despite a lot of public health efforts, many people who infect fungi in the blood die, even if they ... Views: 524
In order to improve the quality of raw materials during the processing and production of Western-style pastry, some additives are often needed to assist the processing in the initial processing stage. However, for different taste needs, if the appropriate selection and dosage adjustment of these ... Views: 524
The growth and reproduction of life, birth and death, and metabolism are all related to enzymes. Without the catalysis of enzymes, humans can't even carry out the most basic life activities, such as food digestion, oxygen breathing, etc., let alone other problems. In fact, almost all kinds of ... Views: 523
As an efficient biocatalyst, enzyme preparations are widely used in the production of food additives. In the 1940s, the microbial enzyme preparation industry became the main source of enzyme preparations due to its wide variety, fast growth, easy processing and purification, and relatively low ... Views: 521
As an essential substance for people's daily life, milk is a nutritious food that provides vitamins, minerals, protein, carbohydrates, as well as depending on the types of milk you drink, some may also have energy-rich fats. However, large amounts of nutrients need to be digested to release ... Views: 520
Enzyme activity assay is an analytical technique that uses the properties of enzymes to specifically and efficiently catalyze chemical reactions, and detects the content and activity of certain enzymes in biological samples such as body fluids by measuring the rate of enzymatic reactions.
1. ... Views: 515
Enzyme is a kind of vital protein that takes charge of many reactions in the organism. Non-protein molecules called coenzymes also help enzymes work. Coenzymes, usually derived from vitamins, are much smaller than the enzymes themselves but equally important. From accelerating digestion to ... Views: 513
Biosensors made with enzymes are becoming more and more widely used in environmental monitoring. Enzymes are playing an important role in the process of waste treatment and recycling. Using genetic engineering and protein engineering to expand the metabolic pathways of enzymes is the treatment ... Views: 512
Among the seven protease families, serine (eg, proteinase K), cysteine, threonine, proteases, aspartic proteases, glutamic proteases, metalloproteases, asparagine peptide lyases play a major catalytic role. Proteases break down long chain molecules of proteins into shorter fragments. They are ... Views: 507
Modern molecular science believes that the normal progress of biological activities depends on the balance and stability of biochemical reactions inside the body. This complex and ordered biochemical reaction requires enzymes to catalyze regulation to maintain normal metabolism in the ... Views: 506
Various biochemical reactions continue to occur in organisms, which require corresponding enzymes to catalyze, so there are many types of enzymes. According to statistics from the enzyme website BRENDA, the number of enzymes officially included has reached 7,984.
To study and apply these ... Views: 505
There are more than 50 kinds of microorganisms (including aerobic bacteria and anaerobic bacteria) living in the vagina of healthy women, and it is also a microbial ecology. Aerobic bacteria include: vaginal bacillus (dominant), corynebacterium, non-hemolytic streptococcus, enterococcus, ... Views: 503
With the continuous advancement of science and technology, the medical community's insight into health, sub-health and disease has also deepened, and a new concept of human disease-genetic disease has been proposed. Genetic damage is the source of all diseases. Because genes cannot be repaired, ... Views: 501
High school textbooks say that most enzymes are proteins. This sentence is not comprehensive, and you can't know this fact just by deduction—enzymes are not only proteins, but also RNases.
What is an enzyme?
Enzymes are defined as macromolecules capable of catalyzing biochemical reactions. ... Views: 496
The pulp and paper industry is one of the important pillar industries of the national economy, but the current pulp and paper industry is faced with the shortage of raw materials, energy shortages, and serious pollution, which restrict its development.
In recent years, people have ... Views: 494
Lipids are hydrocarbon-containing molecules that make up the structure and function of living cells. Lipids include fats, oils, waxes, certain vitamins (such as vitamins A, D, E, and K), hormones, and most cell membranes that are not composed of proteins. Lipids are insoluble in water because ... Views: 493
Metabolism is the basis for all life activities of an organism, and metabolism cannot be achieved without the catalytic action of enzymes. They are catalytic organic substances produced by living cells. Essentially, the vast majority of enzymes are proteins, and a few are RNA, or ribonucleic ... Views: 492
Enzymes are a type of protein present in all living things. They are secreted by the source and are biological catalysts, increasing the rate of chemical reactions without undergoing any permanent change themselves. They continue to work as long as the “substrate” is present.
Enzymes are ... Views: 492
In order to carry out various life activities, the organism must obtain energy and produce new substances needed for its growth. By ingesting nutrients, organisms produce energy through decomposition; or produce new substances through synthesis. In organisms, nutrition molecules participate in ... Views: 490
Although the pancreas is small, it has an extraordinary effect and can be said to be one of the most important organs for mankind. It is important because it is a gland with internal and external secretory functions, and its physiological and pathological changes are closely related to ... Views: 490
The factors that affect enzyme catalysis (that is, enzyme activity) will definitely affect the rate of enzymatic reactions, but the factors that affect the rate of enzymatic reactions do not necessarily affect the catalysis. pH, temperature, ultraviolet light, heavy metal salts, inhibitors, ... Views: 490
Source of xylanase
Xylanases are widely distributed in nature and can be obtained from animals, plants and microorganisms. Xylanase is present in marine and terrestrial bacteria, marine algae, fungi, yeast, rumen and ruminant bacteria, snails, crustaceans, land plant tissues and various ... Views: 489
Alternatively referred to as hydrolases, the hydrolytic enzymes are used to split different groups of biomolecules, like esters, peptides, and glycosides. Moreover, hydrolytic enzymes can break down protein, lipids, nucleic acids, carbohydrates, and fat molecules to their simplest or smallest ... Views: 489
Lipase (EC 3.1.1.3), the full name of triacylglycerol hydrolase, is a class of biological enzymes that can hydrolyze long-chain fatty acid glycerides into fatty acids and monoglycerides, diglycerides or glycerol. It can be derived from animals, plants and microorganisms, and can catalyze ... Views: 488
Currently, the ongoing COVID-19 epidemic has led to an unprecedented shortage of disinfectants, hand sanitizers and detergents, especially in the United States and Europe. Hand sanitizer is regarded as an auxiliary product of hand soap and liquid. Since no vaccines or antiviral drugs have been ... Views: 487
In the early 20th century, humans discovered vitamins, and then trace elements and minerals. It was only in the mid-1980s that humans realized the importance of enzymes. Nobel Prize in Physiology and Medicine Arthur Kornberg said: "For our lives, we can no longer find other substances as ... Views: 485
Enzymes have unparalleled advantages over ordinary catalysts and have been widely used in food, medicine, textile, detergent and cosmetics industries. Most of these applications are performed in aqueous solution, but many valuable products are insoluble in water, and there are many useful ... Views: 484